摘要
采用ＭＴＴ 比色分析法研究极低频磁场对ＳＨ－ＳＹ５Ｙ 和ＰＣ－１２ 瘤细胞代谢活力的影响．实验结果表明：在相同参数的磁场作用下，不同类型细胞代谢活力的变化不同；ＳＨ－ＳＹ５Ｙ 和ＰＣ－１２ 两种瘤细胞都对磁场振幅和频率敏感，在某些磁场参数下磁场照射能够使细胞代谢活力有明显降低或升高；振幅和频率不同的磁场，即使都对细胞代谢活力有影响，磁场作用的大小也存在差异．
The effects of extremely low frequency magnetic fields on the metabolic activity of SH SY5Y and PC 12 tumor cells are studied by MTT colorimetric assays. The experimental results show that the effects of the same magnetic fields on the metabolic activity of different cells (SH SY5Y and PC 12) are different; both of the cells are sensitive to the frequency and the amplitude of magnetic fields and their metabolic activity decreases or increases significantly under the exposure to magnetic fields with the specific frequency and amplitude. The extend of the change of the cell metabolic activity induced by different effective magnetic fields is different. The extremely low frequency magnetic fields under specific frequencies and amplitudes will inhibit or enhance the respiration of the cells.
出处
《华中理工大学学报》
CSCD
北大核心
1999年第9期55-57,60,共4页
Journal of Huazhong University of Science and Technology
基金
国家自然科学基金
关键词
极低频磁场
MTT
比色分析
细胞代谢活力
瘤细胞
extremely low frequency magnetic fields
MTT colorimetric assay
metabolic activity of cells Huang Chunming Doctoral Candidate
Dept. of Bioengineering, HUST, Wuhan 430074, China.
