人类白细胞抗原-DQB1等位基因多态性与我国南方汉族人群多发性硬化的相关性研究

Study of association between human leucocyte antigen-DQB1 allele gene polymorphism and multiple sclerosis in Han population from southern area of China

目的探讨人类白细胞抗原(HLA)-DQB1基因多态性与我国南方汉族人群多发性硬化(MS)的相关性。方法采用基因测序的方法(SBT)对42例南方汉族MS患者及48名健康对照者进行HLA-DQB1等位基因的检测,并比较MS组与健康对照组之间等位基因型频率的差异。结果共检测到15种HLA-DQB1等位基因片段,其中DQB1*0502等位基因频率MS组(35.7%)显著高于健康对照组(8.9%)(P=0.0018,Pc=0.027,OR=4.29);DQB1*0303等位基因频率MS组(19.0%)低于健康对照组(39.6%)(P=0.04,OR=0.48),但差异经校正后无统计学意义。HLA-DQB1*0601和DQB1*0602等位基因频率在MS组患者与健康对照组之间差异无统计学意义。结论我国南方汉族人群MS与HLA-DQB1*0502等位基因有关,而与HLA-DQB1*0601和DQB1*0602等位基因无关。

Objective To approach the association between human leucocyte antigen（HLA）-DQB1 allele gene polymorphism and multiple sclerosis（MS） in Han population from southern area of China.Methods The HLA-DQB1 genotypes of the 42 Han patients with MS（MS group） and 48 healthy controls（healthy control group） all of them from southern area of china were determined by sequencing-based typing（SBT） method.The frequencies of the DQB1 alleles were compared between the two groups.Results A total of 15 DQB1 allele genes were identified.The frequency of the DQB1＊0502 allele gene in the MS group（35.7%） was significantly higher than that in the healthy control group（8.9%）（P=0.0018,Pc=0.027,OR=4.29）.The frequency of the DQB1＊0303 allele gene in the MS group（19.0%） was lower than that in the healthy control group（39.6%）（P=0.04,OR=0.48）,but the difference was no statistical significance since corrected.In addition,the freguencies of HLA-DQB1＊0601 and DQB1＊0602 allele genes were no significantly difference between the MS group and health control group.Conclusion MS in Han population from southern area of China is associated with HLA-DQB1＊0502 allele gene,but not with the DQB1＊0301 and DQB1＊0602 allele genes.