摘要
目的尼妥珠单抗(Nimotuzumab)简称hR3,是表皮生长因子单克隆抗体,对多种实体瘤细胞具有明显的放疗增敏作用,四氧化三铁纳米粒子有良好的磁共振信号敏感性,文中探讨hR3包被的超胜磁性四氧化三铁纳米颗粒(superpara-magnetic iron oxide particles,SPIO)对肺癌A549细胞的放射增敏作用和对肺癌细胞体外磁共振成像(MRI)的影响。方法采用MTT法检测hR3-SPIO对肺癌A549细胞的毒性;用克隆形成抑制实验检测hR3-SPIO对肺癌A549细胞放射增敏的影响,用多靶单击方程拟合肺癌A549细胞的剂量存活曲线,求出放射增敏比(sensitization enhancement ratio,SER),评价增敏效果;将hR3-SPIO、hR3、SPIO分别与A549细胞孵育,同时设立对照组,体外MRI观察各组T2弛豫时间。结果不同浓度的hR3-SPIO作用于人肺癌A549细胞株24 h后,其细胞毒性呈剂量依赖性;hR3-SPIO对肺癌A549细胞有放射增敏作用,25μg/ml hR3-C225培养24 h的放射增敏比(SER)为1.20;体外MRI显示hR3-C225组T2弛豫时间低于其他3组(P<0.05)。结论 hR3-SPIO对肺癌A549细胞有毒性作用和有放射增敏性;hR3-SPIO对表皮生长因子受体过表达的肺癌细胞具有较好磁共振靶向成像作用。
Objective Nimotuzumab（hR3） is an epidermal growth factor of monoclonal antibodies and has a good radiosensitizing effect on many solid tumor cells,particularly the good magnetic resonance imaging signal sensitivity of superparamagnetic iron oxide nanoparticles（SPIO）.This study was to explore the radiosensitizing effect of hR3-coated SPIO（hR3-SPIO） on lung cancer A549 cells.Methods The toxicity of hR3-SPIO on lung cancer A549 cells was determined by MTT,its effect on the radiosensitivity of the cells detected by clonogenic assay,and the sensitization enhancement ratio（SER） calculated with the dose-survival curves.hR3,hR3-SPIO and SPIO were respectively incubated with the A549 cells,and the T2 relaxation time of each group was observed by MR imaging in vitro.Results The cytotoxicity of hR3-SPIO on the lung cancer A549 cells increased in a dose-dependent manner.hR3-SPIO exhibited a radiosensitizing effect on the cells,with an SER of 1.20 after 24 hours of exposure to hR3-C225 at 25 μg/ml.The hR3-C225 group showed a significantly shorter T2 relaxation time than the other groups（P0.05）.Conclusion hR3-SPIO has toxic and radiosensitizing effects on lung cancer A549 cells,and contributes to the targeted MRI of lung cancer cells with the over-expression of the epidermal growth factor receptor.
出处
《医学研究生学报》
CAS
2011年第2期127-130,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金(30970792)
