巨噬细胞抑制因子-1及尿激酶型纤容酶原激活剂在胃癌组织中的表达及临床病理意义

Clinical and pathological significance of MIC-1 and uPA expression in gastric carcinoma

目的:探讨巨噬细胞抑制因子-1(macrophageinhibitory cytokine-1,MIC-1)及尿激酶型纤容酶原激活剂(urokinase plasminogen activator,uPA)在胃癌组织中的蛋白表达及其临床意义.方法:收集郑州大学第一附属医院2009-01/2010-05胃癌存档蜡块55例,采用免疫组织化学方法检测55例胃癌及正常胃黏膜上皮组织中MIC-1及uPA的蛋白表达情况,并分析其表达与胃癌临床病理特征的关系.结果:MIC-1蛋白在胃癌组织中的阳性表达率显著高于正常胃黏膜上皮,组间比较差异均具有统计学意义(56.4%vs20.0%,χ2=7.792,P<0.05),并且其在胃癌组织中的阳性表达率与癌的组织学分级、浸润深度及淋巴结转移均相关(χ2=6.781,11.071,12.806,均P<0.05).uPA蛋白在胃癌组织中的阳性表达率显著高于正常胃黏膜上皮,组间比较差异均具有统计学意义(61.8%vs25.0%,χ2=7.965,P<0.05),并且其在胃癌组织中的阳性表达率与癌的组织学分级、浸润深度及淋巴结转移均相关(χ2=13.803,14.561,10.668,均P<0.05).MIC-1与uPA蛋白表达呈正相关关系(γp=0.591,P<0.05).结论:MIC-1及uPA的过表达可能与胃癌的发生发展有关,并对判断胃癌患者的生物学行为具有一定的意义.

AIM： To investigate the expression of macro- phage inhibitory cytokine-1 （MIC-1） and uro- kinase plasminogen activator （uPA） in gastric carcinoma and to analyze their clinical and pathological significance. METHODS： Fifty-five gastric carcinoma tissue samples obtained from patients treated from Jan 2009 to May 2010 at the First Affiliated Hospital of Zhengzhou University were used to detect MIC-1 and uPA protein expression by immuno- histochemistry. Twenty normal gastric epithelial tissue samples were used as controls. Correla- tion between MIC-1 and uPA expression and clinical and pathological parameters of gastric carcinoma was then analyzed RESULTS： The positive rate of MIC-1 expres- sion in gastric carcinoma was significantly higher than that in normal gastric epithelial tis- sue （56.4% vs 20.0%, ;（2 = 7.792, P 〈 0.05）. The expression of MIC-1 protein was closely corre- lated with tumor grade, infiltration and lymph node metastasis in gastric carcinoma （x2 = 6.781, 11.071, 12.806, all P 〈 0.05）. The positive rate of uPA expression in gastric carcinoma was also significantly higher than that in normal gastric epithelial tissue （61.8% vs 25.0%, ;（2 = 7.965, P 〈 0.05）. The expression of uPA protein was also closely correlated with tumor grade, infiltration and lymph node metastasis in gastric carcinoma （;（2 = 13.803, 14.561, 10.668, all P 〈 0.05）. There was a positive correlation between the expres- sion of MIC-1 and uPA （yp = 0.591, P 〈 0.05）. CONCLUSION＂ The expression of MIC-1 and uPA may be associated with gastric carcinogenesis. Detection of MIC-1 and uPA expression can be used for evaluation of the biological behavior of gastric carcinoma.