新疆伊犁地区牧羊犬脑内亨德拉病毒感染情况

Intracerebral Infection with Hendra Virus in Shepherd Dogs in Ili Prefecture,Xinjiang Uygur Autonomous Region,China

目的分析新疆伊犁地区草原放养牧羊犬脑内亨德拉病毒(Hendravirus,HeV)的感染情况。方法采用一步法实时荧光定量RT-PCR检测HeV核蛋白(N)基因片段,并验证该方法的敏感性和特异性。采用该方法对采自新疆伊犁地区草原放养、未接种HeV疫苗的101例牧羊犬脑组织进行HeV N基因片段检测。结果建立的一步法实时荧光定量RT-PCR检测HeV N基因的最低检出浓度为2.6×102 copies/μl;与其他单股负链RNA病毒,如同属且高度同源的尼帕病毒(Nipah virus,NiV)和博尔纳病病毒(Borna disease virus,BDV)等无交叉反应;101份牧羊犬脑组织样本HeV N基因的检测结果均为阴性。结论尚未发现我国新疆伊犁地区天然牧场放养牧羊犬中存在HeV感染的证据,该地区短时间内爆发HeV感染的危险性不大。

Objective To investigate the intracerebral infection with Hendra virus（HeV） in shepherd dogs in Ili Prefecture,Xinjiang Uygur Autonomous Region,China.Methods A one-step real-time fluorescent quantitative RT-PCR method for nucleoprotein（N） gene fragment of HeV was developed,verified for sensitivity and specificity,and used for determination of HeV N gene in 101 brain tissue samples of uninoculated shepherd dogs bred in grassland in Ili.Results The minimum detection limit of HeV N gene by the developed method was 2.6 × 102 copies / μl.No cross reactions with other single negative strand RNA virus such as Nipah virus（NiV） were observed.All the detection results of 101 brain tissue samples of shepherd dogs were negative.Conclusion There is no evidence of HeV infection in the shepherd dogs bred in natural grazing lands in Ili,suggesting low risk of outbreak of HeV infection in the near future.