摘要
目的观察缺血后适应对肢体缺血再灌注(LIR)后肺损伤的影响。方法实验采用大鼠肢体缺血再灌注损伤模型,将Wistar大鼠随机分三组,即:对照组、单纯缺血再灌组、缺血后适应组,分别测定血浆及肺组织中与自由基有关物质的含量,光镜下观察肺组织形态学变化。结果缺血后适应可有效地降低自由基生成酶活性,增加自由基清除酶的活性,抑制组织脂质过氧化的发生,减轻组织水肿及中性粒细胞的聚集。结论缺血后适应对大鼠肢体缺血再灌注后继发的肺损伤具有保护作用,其机制可能与其减少自由基的生成,增强抗氧化能力有关。
Objective On the model of rats＇ limb ischemia-reperfusion（LIR）,the effect of postconditioning on the lung injury was observed.Methods Using wistar rats.Animals were randomly divided into three groups：control group,LIR group and IPo group.The content of materials related to free radicals in the plasma and lung tissue were measured.The morphologic changes of the lung tissues were observed under microscope.Results It was found that postconditioning significantly decreased the activity of produced free radicals from cytoplasma,increased the activity of the enzyme deleted free radical,inhibited the formation of tissue lipid peroxide,alleviated the edema of tissue and the neutrophil infiltrated.Conclusion Ischemic postconditioning protected the lung from the limb through decreasing the produce of radical and increasing the ability of oxidation resistance.
出处
《中国煤炭工业医学杂志》
2011年第2期255-257,共3页
Chinese Journal of Coal Industry Medicine
关键词
缺血再灌注
肺损伤
后适应
肢体
大鼠
Ischemia reperfusion
Lung injury
Postconditioning
Limb
Rats
