罗格列酮对肥胖糖尿病小鼠脂肪组织中Perilipin基因表达的影响

Effects of rosiglitazone on the expression of perilipin mRNA in adipose tissue of obese diabetic mice

目的观察罗格列酮(RGZ)对肥胖糖尿病小鼠脂肪组织中脂滴包被蛋白(Perilipin)表达的影响,并探讨其作用机制。方法 36只雄性C57BL/6小鼠随机分为对照(NC)组、糖尿病(DM)组及干预(RGZ)组,建立肥胖糖尿病小鼠模型,并应用RT-PCR检测各组小鼠脂肪组织中Perilipin基因的表达水平。结果与NC组比较,DM组小鼠脂肪组织中Perilipin基因表达水平明显下调(P<0.01)。而经RGZ干预后,RGZ组小鼠脂肪组织中Perilipin基因表达水平明显高于DM组(P<0.01)。结论 RGZ能上调肥胖糖尿病小鼠脂肪组织中Perilipin基因表达,并调节脂代谢紊乱及改善胰岛素抵抗。

Objective To observe the effects of rosiglitazone on the expression of perilipin mRNA in adipose tissue of obese diabetic mice. Methods 36 male C57BL/6 mice were randomly divided into control （NC） group, diabetes mellitus （DM） group, and rosiglitazone intervention （RGZ） group. Then obese and diabetic mice model were established, and the expression levels of perilipin mRNA were detected by RT-PCR. Results The expression of perilipin mRNA in adipose tissue of mice was lower in DM group than in NC group [（0. 48±0.12） vs （1.08±0.09）, P〈0.01] and was higher in RGZ group than in DM group [（0. 81±0.10） vs （0.48±0.12） ,P〈0.01]. Conclusions PPARγ agonist rosiglitazone may up-regulate the gene expression of perilipin in adipose tissue of obese diabetic mice, regulate lipid metabolism and improve insulin resistance.