木芙蓉药材HPLC指纹图谱研究

Fingerprints of Hibiscus Mutabilis by HPLC

目的:采用高效液相色谱法建立木芙蓉药材的指纹图谱,为其品质控制提供依据。方法:采用Waters Xbridge C18(4.6 mm×250 mm,5μm)色谱柱;流动相乙腈-1%冰乙酸水溶液梯度洗脱;检测波长370 nm;柱温25℃。结果:10批各产地的木芙蓉药材指纹图谱峰有10个共有峰,峰面积之和大于总峰面积的90%,与对照品比较共有峰中检出芦丁、金丝桃苷、槲皮苷。结论:该方法准确可靠,为木芙蓉药材及其制剂质量控制提供了依据。

Objective：To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Hibiscus Mutabilis.Method：HPLC method was set up by Waters Xbridge C18column（4.6 mm × 250 mm,5 μm） with gradient elution;the mobile phase was comprised of acetonitrile-1% acetic acid solution,the UV detection wavelength was at 370 nm with a flow rate of 1.0 mL·min^-1,and column temperature was at 25 ℃.Result：There were 10 common peaks in the fingerprints of H.mutabilis based on 10 habitats.Sum of peak areas was larger than 90% of total area.As compared with standard sample,rutin,hyperin and quercitrin were checked out.Conclusion：The method is reliable,accurate and can be used as a quality control method for H.mutabilis.