云南昭通天麻松弛血管平滑肌活性成分的筛选

Screening of Active Compounds from Gastrodia elata Blume for Vascular Smooth Muscle Relaxation

目的:观察天麻血管平滑肌松弛作用,明确其作用物质基础。方法:采用大鼠离体胸主动脉环灌流实验方法,对天麻血管平滑肌松弛作用进行考察,采用柱色谱法、光谱法(MS,NMR)对其活性成分进行了分离鉴定。结果:天麻能够显著抑制KCl引起的大鼠胸主动脉环收缩,从天麻乙酸乙酯提取物中分离并鉴定了5个酯溶性酚性成分(Ⅰ～Ⅴ):对羟基苯甲醛(Ⅰ)、对羟苄基甲醚(Ⅱ)、对羟基苯甲醇(Ⅲ)、4,4′-二羟基二苯基甲烷(Ⅳ)、4,4′-二羟基二苄醚(Ⅴ),明确了天麻的血管平滑肌松弛作用是这5个成分共同作用的结果。结论:天麻具有显著的血管平滑肌松弛作用,其作用主要由5个酯溶性酚性成分共同发挥。

Objective：To study the vascular smooth muscle relaxation effect and explicit the material base of Gastrodia elata Blume.Method：Tension recording for rat isolated aortic artery was used to study the effect of vascular smooth muscle relaxation.Column chromatography and mass spectrography and nuclear magnetic resonance spectrometry were used to isolate and identify the active compounds of G.elata Blume.Result：G.elata Blume.could significantly inhibit the smooth muscle constriction of isolated aortic artery induced by KCl.Five esterified phenolic compounds（Ⅰ-Ⅴ） were isolated from G.elata Blume,such as p-hydroxybenz aldehyde（Ⅰ）;p-hydroxybenzyl methylether（Ⅱ）;p-hydroxybenzyl alcohol（Ⅲ）;4,4′-dihydroxydiphenyl methane（Ⅳ）;4,4′-dihydroxydibenzyl ether（Ⅴ）.The results showed that the vascular smooth muscle relaxation effects were the result of the role of five compounds.Conclusion：The five esterified phenolic compounds（Ⅰ-Ⅴ） from G.elata Blume.play a combined role for vascular smooth muscle relaxation.