草苁蓉联合长春瑞滨化疗对VX2移植瘤的抑瘤作用及机制研究

Inhibitory Effect and Mechanism of Boschniakia rossica Combined with Vinorelbine Chemotherapy on Growth of VX2 Transplanted Tumor

目的:探讨草苁蓉提取物(BRE)联合长春瑞滨(NVB)化疗对兔VX2移植瘤的治疗作用和抗氧化能力的影响。方法:建立兔VX2皮下移植瘤模型,将其分为模型组,BRE组,NVB组和BRE+NVB组(BRE和NVB联合组)。于造模第8天开始给药,BRE组和BRE+NVB组分别ig给药100 mg.(kg.d)-1 BRE共21 d,NVB组和BRE+NVB组于造模第15,22天静脉注射2.5 mg·kg-1.d-1 NVB共2次。观察各组荷瘤兔的肿瘤生长情况。实验结束后,称瘤重和计算抑瘤率。提取瘤体标本,免疫组化法检测移植瘤细胞增殖细胞核抗原(PCNA)表达情况。比色法检测血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力(TAOC)和丙二醛(MDA)含量。结果:各治疗组对移植瘤生长和肿瘤细胞增殖有明显的抑制作用,其中BRE+NVB组肿瘤生长抑制率最高、增殖指数最小。同时,NVB虽明显增高血清CAT,GSH-Px活性,但SOD活性和TAOC水平显著降低,MDA水平明显增高。而BRE组和BRE+NVB组均可明显升高血清SOD,CAT,GSH-Px活性和TAOC水平,降低MDA水平。结论:BRE对VX2移植瘤具有明显的抑制作用,且对长春瑞滨化疗有明显增强作用。其作用可能与其抑制细胞增殖和增高机体抗氧化能力有关。

Objective：To investigate the therapeutic and antioxidative effect of Boschniakia rossica extract（BRE） combined with vinorelbine（NVB） chemotherapy in VX2-bearing rabbits.Method：The VX2 transplanted rabbit model was established and the rabbits were divided into 4 groups：model,BRE,NVB,BRE ＋ NVB（BRE combined with NVB）.Animals in BRE and BRE ＋ NVB groups were treated with 100 mg·kg^-1.d-1 BRE i.g.for 21 days from the 8th day,and animals in NVB and BRE ＋ NVB groups were treated with 2.5 mg·kg^-1.d-1 NVB i.v.twice on the 15th and 22st day.Then the size and weight of tumor were estimated and the growth inhibition ratio was calculated.The expression of proliferation cell nuclear antigen（PCNA） protein was determined with immunohistochemical method,and the serum activities of superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-Px）,total antioxidant activity（TAOC）,as well as the content of malondialdehyde（MDA） were detected by colorimetric method.Result：The administration with BRE and/or NVB inhibited the growth of transplanted tumor and the proliferation of tumor cell,while the BRE ＋ NVB group showed the highest growth inhibition ratio and the lowest proliferation index.Furthermore,NVB treatment increased serum MDA content and reduced serum levels of SOD and TAOC despite of the increased activities of CAT and GSH-Px.However,BRE or BRE ＋ NVB treatment increased the serum SOD,CAT,GSH-Px and TAOC,and reduced the serum MDA level in VX2-bearing rabbits.Conclusion：BRE exhibits an inhibitory effect on the growth of transplanted VX2 tumor,and enhances the therapeutic effect of NVB chemotherapy,probably via the inhibition of tumor cell proliferation and the improved antioxidative capability.