蒺藜提取物对阿霉素所致大鼠心肌细胞凋亡的影响

Effect of Extract from Tribulus terrestris on Myocardial Cell Apoptosis Caused by Adriamycin in Rats

目的:研究蒺藜提取物对阿霉素(ADR)所致大鼠心肌细胞凋亡的影响。方法:将蒺藜提取物灌胃于ADR所致心肌细胞凋亡的大鼠,采用电镜对大鼠心肌细胞进行超微结构观察,采用TUNEL法检测心肌细胞凋亡指数,免疫组化SP法检测心肌细胞Bax,Bcl-2蛋白的表达。结果:ADR累积用量20 mg·kg-1可致大鼠心肌细胞凋亡,蒺藜提取物中、高剂量组(40,60 mg·kg-1)可减轻心肌细胞超微结构的损伤,蒺藜提取物中、高剂量组较模型组均可显著降低细胞凋亡率(P<0.05)。蒺藜提取物中、高剂量组可显著上调Bcl-2表达(P<0.01)、下调Bax表达(P<0.05)。结论:蒺藜提取物可抑制ADR所致大鼠心肌细胞的凋亡,对阿霉素所致的心脏毒性有保护作用。

Objective：To investigate the effect of extract from Tribulus terrestris on myocardial cell apoptosis caused by adriamycin in rats.Method：Rats were given intraperitoneal injection of adriamycin（ADR） to induce cardiocyte apoptosis.After continuous ig of extract from T.terrestris,myocardial ultrastructure in every experimental group was observed with electron microscope.The apoptotic rate of myocardial cells was determined by terminal deoxynucleotidyl transferaseediated dUTP nick-end labeling（TUNEL）,and the expression of Bcl-2 and Bax was detected by using immuno-histochemical method.Result：ADR of 20 mg·kg^-1 induced cardiocyte apoptosis.Extract from T.terrestris middle-and high-dosage groups（40,60 mg·kg^-1） could reduce cardiotoxicity injury by ADR.Extract from T.terrestris middle-and high-dosage groups could significantly increase the Bcl-2 expression（P 〈0.01）,reduced Bax expression（P 〈 0.05）.Conclusion：Extract from T.terrestris may decrease the cardiocyte apoptosis induced by adriamycin,and it has a protective effect on adriamycin-induced cardio-toxicity.