多药耐药鲍氏不动杆菌整合子研究

Study on integron in multi-drug resistant acinetobacter baumannii

目的调查多药耐药鲍氏不动杆菌整合子的分布。方法运用PCR扩增多药耐药鲍氏不动杆菌Ⅰ、Ⅱ、Ⅲ类整合子,然后进行整合子可变区序列测定。结果 2007年1-12月共分离到非重复多药耐药鲍氏不动杆菌133株,Ⅰ类整合子检出率为61.9%,未检出Ⅱ、Ⅲ类整合子,成功扩增出81株多药耐药鲍氏不动杆菌整合子的可变区,其中32株携带的基因盒顺序为aacA4-catB8-aadA1,44株为同时含有aacA4-catB8-aadA1和aacC1-orfP-orfQ-aadA1,3株为aacC1-orfP-orfQ-aadA1。结论Ⅰ类整合子与鲍氏不动杆菌耐药性密切相关,在多药耐药鲍氏不动杆菌耐药机制中起重要作用。

OBJECTIVE To investigate the distribution of integrons in multi-drug resistant Acinetobacter baumannii isolates.METHODS PCR was used to screen the class 1,2 and 3 integrons in multi-resistant baumannii isolates,and the sequences of the variable regions of class I integrons were analyzed.RESULTS A total of 133 strains of multi-drug resistant A.baumannii were isolated from Jan to Dec 2007.The detection rate of class I integron in 133 strains was 61.9%.No class Ⅱand Ⅲ integrons were detected.The variable regions of 81 class I integron positive strains were successfully amplified.The gene cassette arrays of 32 strains were aacA4-catB8-aadA1,44 strains were aacA4-catB8-aadA1 and aacC1-orfP-orfQ-aadA1 together and 3 strains were aacC1-orfP-orfQ-aadA1.CONCLUSIONS Class I integron is closely related to the resistance of A.baumannii and plays an important role in multi-drug resistance.