酒花黄酮提取工艺和含量测定

Optimization of Extraction Process and Content Determination of Total Flavonoids in Humulus lupulus L.

建立酒花黄酮的提取方法和含量测定方法。采用NaNO2-Al(NO3)3-NaOH显色法,以芦丁为对照品,通过单因素试验和L9(34)正交设计确定酒花黄酮的提取方法,并测定和比较酒花、酒糟和啤酒中黄酮的含量。结果表明:酒花黄酮提取方法为样品加入50倍的甲醇,超声提取2次,每次1h,酒花黄酮含量为69.98mg/g,酒糟和啤酒中黄酮的含量较低。对照品芦丁在10.2～40.8μg/mL质量浓度范围内与吸光度呈良好线性关系,平均回收率99.33%,RSD=3.60%。该提取方法合理,含量测定方法简便、快速、准确,可用于酒花黄酮提取物制备及其质量控制。

Ultrasonic extraction conditions for total flavonoids from Humulus lupulus L.were optimized by single factor experiments and orthogonal design with the aim of establishing a sample preparation method for determination of the content of total flavonoids in Humulus lupulus L.by NaNO2-Al（NO3）3-NaOH colorimetric method using rutin as the reference.The optimal extraction process parameters were material-to-liquid ratio of 1：50,extraction duration of 1 h and repeated extraction number of 2.The content of total flavonoids from Humulus lupulus L.was 69.98 mg/g under the optimal extraction conditions,which was much higher than that of residues and beer.The linear range was between 10.2μg/mL and 40.8μg/mL with a correlation coefficient of 0.9985.The average recovery rate was 99.33% with a relative standard deviation of 3.60%.The extraction and determination methods were simple and feasible,which is suitable for the preparation and quality control of flavonoids extracted from Humulus lupulus L.