摘要
目的:建立衍生分光光度法测定麦麸粗提液中阿魏酸的含量。方法:以0.6g/100mL FeCl3-0.9g/100mLK3[Fe(CN)6](1:0.9)混合溶液为显色剂,并以显色剂为空白,在720nm波长处测定吸光度。结果:阿魏酸在2.36~14.16μg/mL范围内,吸光度与阿魏酸的质量浓度呈良好的线性关系(r=0.9996),最小检出量为0.022μg/mL,平均加标回收率为103.1%。结论:本检测方法操作简便、准确、回收率高,适用于麦麸粗提液中阿魏酸含量的测定。
Objective:To establish a determination method of ferulic acid in wheat bran by derivatization combined with spectrophotometry.Methods:Ferulic acid was analyzed spectrophotometrically at 720 nm.The color-developing agent was the mixture of 0.6 g/100 mL FeCl3 and 0.9 g/100 mL K3Fe(CN)6](1:0.9).Results:A good linear relationship in the concentration range of 2.36-14.16μg/mL for ferulic acid(r = 0.9996) was observed,and the detection limit of this developed method was 0.022μg/mL.The average recovery rate of this method was 103.1%.Conclusion:This developed method is characteristics of simple operation,accurate results and high average recovery rate,which is suitable for the determination of ferulic acid in wheat bran.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第6期155-158,共4页
Food Science
基金
教育部大学生创新性实验项目(091062627)
四川省人事厅留学人员择优项目(川人函【2008】488号)
四川省教育厅重点项目(09ZA081)
关键词
麦麸
阿魏酸
衍生分光光度法
wheat bran
ferulic acid
derived spectrophotometry