高效液相色谱法测定水果中吡虫啉农药残留量

Determination of Imidaclprid Residue in Fruits by HPLC

建立高效液相色谱法测定水果中的吡虫啉残留量的检测方法。采用改进的QuEChERS方法,乙腈匀浆提取,提取液加C18和PSA填料净化,液相色谱法检测。结果表明:方法的定量检测限为0.05mg/kg,标准曲线线性相关系数0.9988;当添加水平在0.05～0.20mg/kg范围内时,回收率均可在92%～102%之间,相对标准偏差在5%～11%之间。本方法是一种快速、准确、灵敏度高的同时检测水果中吡虫啉残留的检测方法。

A high performance liquid chromatography（HPLC） method was developed to determine imidaclprid residue in fruits.Samples were extracted by the improved QuEChERS method using acetonitrile as the solvent and purified on C18/PSA column.The detection limit of this developed HPLC method was 0.05 mg/kg and the linear correlation coefficient of standard curve was 0.9988.Recovery rates for imidaclprid in spiked samples at 0.05,0.1 mg/kg and 0.20 mg/kg was in the range of 92%-102% with a relative standard deviation varying from 5% to 11%.Therefore,this developed method is characteristics of rapid determination,high accuracy and sensitivity so that it is suitable for the detection of imidaclprid residue in fruits.