摘要
建立快速分离液相-四级杆飞行时间串联质谱(RRLC-Q-TOF)联用技术分析大豆中的异黄酮的方法。大豆样品经甲醇超声辅助提取后,选用Welch Materials C18柱,以乙腈-水(含0.1%甲酸)为流动相进行梯度洗脱。结果表明在正离子模式下,经快速分离液相-四级杆飞行时间串联质谱分离并检测出9种异黄酮成分。RRLC-Q-TOF联用技术可以快速准确鉴定样品中的异黄酮成分。
A novel method for the analysis of isoflavonoids in soybean was established by rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry（RRLC-Q-TOF-MS）.Samples were extracted with methanol under ultrasound assistance,and then a Welch Materials C18 column was applied in the RRLC separation using acetonitrile and water（0.1% formic acid） as the mobile phase.Elutes were detected by Q-TOF to obtain MS spectra with a positive ion mode.Nine isoflavonoids were identified.Therefore,this method can be used to rapidly determine isoflavonoids of soybean.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第6期233-236,共4页
Food Science
基金
国家自然科学基金项目(20872083)
山东省科技攻关项目(2009GG2001021-16)
作物生物学国家重点实验室开放课题(20082KF05)
