丹参提取物对小鼠吗啡身体依赖性形成的影响及其机制

Effect of Salvia miltiorrhiza extracts on formation of morphine physical dependence in mice and its possible mechanism

目的观察丹参提取物(含隐丹参酮85.11%)对小鼠吗啡身体依赖性形成及其对脑内环磷酸腺苷(cAMP)和一氧化氮(NO)生成的影响。方法剂量递增sc吗啡建立小鼠吗啡依赖模型,观察丹参提取物对盐酸纳洛酮催促吗啡依赖小鼠戒断症状的影响,并分别用放射免疫分析法、硝酸还原酶法测定小鼠脑内cAMP和NO水平。结果丹参提取物(80 mg/kg)可显著降低吗啡依赖小鼠催促戒断症状;对吗啡依赖小鼠脑内cAMP无明显影响,但可显著降低吗啡依赖小鼠脑内NO水平。结论丹参提取物能缓解吗啡依赖小鼠的戒断症状,其机制可能与下调脑内NO水平有关。

Objective To observe the action of Salvia miltiorrhiza extracts （SINE2, liposoluble constituents） on the formation of physical dependence induced by morphine in mice and the effects of SINE2 on the production of cyclic adenosine monophosphate （cAMP） and nitric oxide （NO） in brain of morphine-dependent mice. Methods Morphine-dependent mice model was established by sc injection of morphine at a gradual increasing dosage. The effect of SmE2 on the withdrawal symptoms induced by Naloxone in morphine-dependent mice was observed, cAMP and NO content in brain were determined through radioimmunoassay and nitrate reductase, respectively. Results SmE2 （80 mg/kg） could remarkably reduce the withdrawal symptoms in morphine-dependent mice. The cAMP concentration in brain showed no obvious change in morphine-dependent mice treated with SmE2, but NO content in brain could significantly be reduced. Conclusion SmE2 has effect on relieving the withdrawal symptoms in morphine-dependent mice with down-regulation of NO content in brain as possible mechanism.