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亚麻木酚素对肺癌A549细胞的抑制作用及其机制 被引量：4

Inhibition of secoisolariciresinol diglucoside on lung cancer cell line A549 and its mechanism
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摘要 目的观察亚麻木酚素(SDG)对肺癌A549细胞增殖、侵袭和凋亡的影响及机制。方法在体外培养的肺癌A549细胞株中加入不同浓度的SDG(5、10、20、40μmol/L),MTT观察细胞增殖情况;Transwell小室评价细胞侵袭能力;Hochest染色观察细胞凋亡情况;比色法检测半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)活性;Real Time PCR、Western Blotting检测基质金属蛋白酶-2(MMP-2)mRNA和蛋白表达。结果 SDG呈时间-浓度依赖性抑制A549细胞的增殖(P<0.05)。细胞侵袭能力明显下降、凋亡比率和Caspase-3活性明显升高、MMP-2 mRNA和蛋白的表达显著下降(P<0.05)。结论 SDG呈时间和剂量依赖性抑制A549细胞的增殖和侵袭,其机制可能与其促凋亡、抑制MMP-2的表达有关。
作者 李先伟 唐丽娟 杨解人
机构地区 皖南医学院药理学教研室
出处 《中草药》 CAS CSCD 北大核心 2011年第3期550-554,共5页 Chinese Traditional and Herbal Drugs
关键词 亚麻木酚素 肺癌A549细胞 半胱氨酸天冬氨酸蛋白酶-3 基质金属蛋白酶-2 侵袭能力 secoisolariciresinol diglucoside （SDG） lung cancer cell line A549 Caspase-3 MMP-2 invasion ability
分类号 R285.5 [医药卫生—中药学]
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参考文献7

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二级参考文献8

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共引文献4

同被引文献42

  • 1向海艳,周春山,杜邵龙.大孔吸附树脂法分离纯化虎杖中白藜芦醇的研究[J].中草药,2005,36(2):207-210. 被引量：14
  • 2徐晓云,潘思轶,谢笔钧,杨尔宁,陈然.树脂法精制沙棘籽原花色素的研究[J].农业工程学报,2005,21(1):152-154. 被引量：9
  • 3张红,童明容,潘继伦,俞耀庭,王永耀,黄杏林,黄加斗,胡雄军.大孔吸附树脂提取喜树碱的研究[J].离子交换与吸附,1995,11(2):145-150. 被引量：43
  • 4张金.胡麻籽的营养保健价值与产业前景[J].中国食品工业,2006(3):32-32. 被引量：31
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中草药
2011年 第3期

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