脂肪酶催化大豆卵磷脂和亚油酸酯交换反应的研究

Lipase catalyzed transesterification of soybean phosphatidylcholine and linoleic acid

采用Lipozyme TL IM酶催化大豆卵磷脂(90%)和亚油酸(95%)进行酯交换反应,以制备富含亚油酸的卵磷脂。探讨了大豆卵磷脂质量浓度(甲苯为溶剂)、底物摩尔比(n(亚油酸)∶n(大豆卵磷脂))、酶添加量(以底物总质量为基准)、水分添加量(以酶质量为基准)、反应时间、反应温度对酯交换反应的影响。优化后的反应条件为:大豆卵磷脂质量浓度0.25 g/mL,底物摩尔比6∶1,酶添加量20%,水分添加量3%,反应时间60 h,反应温度65℃。在最优反应条件下最终获得82.85%亚油酸含量的卵磷脂产品。

Immobilized lipase Lipozyme TL IM - catalyzed transesterification of soybean phosphatidylcholine（PC, 90% ） with linoleie acid（ LA, 95% ） was carried out in toluene. The six major factors of phosphatidyleholine concentration （toluene as a solvent）, substrate ratio （molar ratio of LA to PC）, lipase dosage （based on the mass of substrates）, water addition （based on the mass of lipase）, reaction time and reaction temperature were discussed. The optimum reaction conditions were as follows： PC concentra- tion O. 25 g/mL, molar ratio of LA to PC 6： 1, lipase dosage 20% , water addition 3% , 65℃ and 60 h. Under the optimum conditions,the maximum content of LA in the prouduet was 82.85%.