摘要
采用HPLC测定芝麻中芝麻素和芝麻林素含量,对超声波提取芝麻中芝麻素和芝麻林素的条件进行了研究,同时探讨了提取液净化处理对其测定结果的影响。通过单因素及正交试验,确定超声波最佳提取条件为:提取剂V(正己烷)/V(甲醇)=60∶40,液料比30∶1,超声提取温度65℃,超声提取时间12 min。采用C18对样品进行净化处理,可提高净化效果,减少测定干扰。
The content of sesamin and sesamolin in sesame were determined by HPLC,and the conditions of the ultrasonic - assisted extraction of the two substances were studied. By the single factor experiments and orthogonal experiments, the extraction conditions were optimized as follows ： n - hexane - MeOH （ volume ratio 60： 40） as extraction solvent, ratio of liquid to material 30： 1, extraction temperature 65 ℃, ultrasonic extraction time 12 rain. The C18 adopted in the pre - processing of sample could enhance the purification effect and decrease the inspectional molestation.
出处
《中国油脂》
CAS
CSCD
北大核心
2011年第3期71-74,共4页
China Oils and Fats
基金
国家芝麻产业技术体系(nycytx-20-1-05)
农业部948项目(2010-C3)
