褐多孔菌发酵培养基优化

Optimization of Liquid Fermentation Condition of Polyporus picipes

以PDA培养基为基础,采用单因子试验方法,对最佳碳源、氮源、培养时间和培养基装液量进行筛选,并采用Box-Benhnken中心组合设计和响应面法对其最佳配比进行优化。结果表明,采用改良的PDA液体培养基(葡萄糖马铃薯液体培养基+酵母粉5.37 g/L+葡萄糖26.31 g/L),褐多孔菌在培养液装液量为每250 mL中装入60.74 mL,经28℃,150 r/min旋转振荡培养68.32 h,次生产物对小麦赤霉的抑制作用最强。培养基优化后褐多孔菌的抗菌活性显著提高,表明响应面法在培养基优化中十分有效,相对简单,且节省时间和材料。

In order to distill and separate the antibacterial active of Polyporus picipes and lay the foundation of application and development of biochemical pesticides,the fermentation medium of Polyporus picipes was optimized by response surface methodology which enhance the activity of antibiotics.PDA was employed as an original medium,and the best carbon,nitrogen sources,culture time and medium volume were identified using single factor approach.The optimized conditions was obtained by the methods of Box-Benhnken central composite design and response surface methodology.Results indicate the strongest antifungal activity of metabolites from Polyporus picipes was obtained when it was cultured in the improved liquid medium based on PDA（liquid medium based on PDA,glucose 26.31 g/L and yeast extract 5.37 g/L） with initial pH and the size of 60.74 mL per 250 mL,Erlenmeyer flask at 150 r/min and 28 for 68.32 h.The results were showed the chosen method of optimization of medium composition was efficient,relatively simple as well as time and material saving.