用于PCR检测的扩展青霉基因组DNA提取方法比较

Comparison of Methods of Genomic DNA Extraction from Penicillium expansum for PCR Detection

为寻找一种经济、简便、高效的基因组DNA提取方法,进一步开展扩展青霉的分子生物学研究,采用氯化苄法、玻璃珠法、玻璃珠+氯化苄法、试剂盒法4种方法提取扩展青霉的基因组DNA,并测定DNA质量浓度以及进行PCR和电泳分析,比较不同提取方法的效果。结果表明,4种方法均可提取到基因组DNA,其抽提质量优劣依次为试剂盒法、玻璃珠+氯化苄法、玻璃珠法、氯化苄法。其中玻璃珠+氯化苄法提取效率接近试剂盒法,效果良好,并且此法成本低廉、操作简单、结果稳定,可代替昂贵的试剂盒法用于扩展青霉基因组DNA的提取。

The aim was to search an economic,convenient and high efficient extraction method for genomic DNA of Penicillium expansum to provide the scientific basis for further molecular biological study.The genomic DNA of Penicillium expansum strains were extracted by four methods such as Benzyl chloride,Glass beads,Glass beads with Benzyl chloride and ZR Fungal DNA Kit,and the effects of the different extraction methods were compared through the quality detection and PCR analysis.The results shows that these four extraction methods all could extract the genomic DNA and their order of the extraction quality from good to bad was ZR Fungal DNA Kit,Glass beads with Benzyl chloride,Glass beads method,Benzyl chloride method,and the method of Glass beads with Benzyl chloride is simple and accurate with a good reproducibility,which can replace the Kit for the extraction of genomic DNA from Penicillium expansum.