HPLC法测定复方逍遥合剂中黄芩苷的含量

Content determination of Baicalin for Compound Xiaoyao Oral Solution by HPLC

目的:建立复方逍遥合剂中黄芩苷的含量测定方法。方法:采用HPLC法,色谱柱Calesil-C18(4.6 mm×250 mm,5μm),流动相:甲醇∶0.1%磷酸(47∶53),检测波长:280 nm。结果:黄芩苷进样量在0.08～0.80μg范围内呈良好的线性关系(r=0.999 9),低、中、高三种浓度的平均回收率为99.59%、100.22%、100.40%,RSD分别为0.75%、2.58%、0.86%。结论:本法简便、准确,重复性好,可用于该产品的质量控制。

Objective： To establish a method for determination the content of Baicalin in Compound Xiaoyao Oral Solution. Methods： Calesil-C18 column （4.6 mm×250 ram, 5 μm） was used, Mobile phase was methanol-0.1% phosphoric acid （47： 53）, Detection wavelength was 280 nm. Results： Baicalin sample size located 0.08-0.80 μg,and favourable linear correlation （r=0.999 9） was showed. The average recovery rates of low, moderate and high concentration were 99.59%, 100.22%, 100.40% with RSD of 0.75%, 2.58%, 0.86%, respectively. Conclusion： The method is convenient and accurate. Meanwhile, it also has good reproducibility, may be used to quality control of Baicalin.