摘要
选择泥鳅(Misgurnus anguillicaudatus)作为受试生物,以泥鳅死亡率、血液红细胞数量、红细胞体积、红细胞微核率和核异常率作为测试指标,考察了沉积物中"加标"金属镉(Cd)的毒性状况,探讨了水体沉积物中重金属的生物毒性影响。结果表明:高有机质的粘土粉砂性沉积物能够有效吸附重金属镉;镉加标暴露组泥鳅的死亡率以及红细胞微核率和核异常率均显著高于对照组(p<0.05),而且各测试指标随着沉积物中镉"加标"浓度的增加而上升。经过计算,水体-沉积物体系中的镉对泥鳅24、48、72和96h LC50分别为2.8037、2.4997、2.2705和1.7538mg.g-1干重。而泥鳅微核率和核异常率对沉积物中的镉的毒性反应灵敏,可以作为监测环境污染的指标。
In order to reflect the toxicity of cadmium(Cd) in the water-sediment system,the toxicological parameters including mortality,micronucleus and nuclear anomalies in erythrocytes of loach(Misgurnus anguillicaudatus) after exposure were quantified.Results show that sediment mainly composed of clay and silt with high content of organic matter could effectively adsorb the spiking cadmium.The mortality and the frequency of micronucleated erythrocytes and the rate of nuclear anomalies of the loans exposed to sediment after cadmium spiking were all significantly higher than those in control(p0.05).According to the data analysis,the median lethal concentrations of cadmium in the water-sediment system were 2.8037,2.4997,2.2705 and 1.7538 mg·g-1 dry weight at 24,48,72 and 96 hours,respectively.The frequency of micronuclei and nuclear anomalies were sensitive to ambient pollutants and could be used to monitor the aquatic environmental quality.
出处
《生态毒理学报》
CAS
CSCD
2011年第1期80-86,共7页
Asian Journal of Ecotoxicology
基金
国家水体污染控制与治理科技重大专项(2009ZX07528-002-02)
关键词
沉积物
泥鳅
镉
死亡率
红细胞
微核
核异常
sediment
loach
cadmium
mortality
erythrocyte
micronucleus
nuclear anomalies