中西药体外抑制幽门螺杆菌效果观察

Analysis of the effects of traditional Chinese medicine and Western medicine inhibit on Helicobacter pylori

目的探讨苦参、金银花、胃康和阿莫西林对幽门螺杆菌(Hp)的抑菌作用。方法取Hp标准菌株(SS1,NT2)菌液各1μl,分别接种于含不同浓度的苦参、金银花、胃康和阿莫西林血琼脂培养皿中,37℃培养72 h后观察最低抑菌浓度(MIC)。结果 4种药物对Hp SS1、NT2的MIC阿莫西林为16和32μg/ml,胃康均为1 000μg/ml,金银花为12 500和6 250μg/ml,苦参均>25 000μg/ml。结论直接抑制和杀灭Hp效果西药优于中药。中西药联用可提高Hp根除率。

Objective To examine the inhibition of 4 kinds of medicines onHelicobacter pylori.Methods One μl ofH.pyloristrains SS1 and NT2 was added to blood agar plates with different concentrations of a decoction of Sophora flavescens,a decoction of Honeysuckle,Weikang capsules,and Amoxicillin capsules.Plates with the 2 strains were incubated at 37 ℃ for 72 hours and then the minimum inhibitory concentration（MIC） was observed.Results In terms of antibacterial effects,Amoxicillin capsules had a MIC with respect to SS1 and NT2 strains of H.pyloriof 16 μg/ml and 32 μg/ml.Weikang capsules had a MIC with respect to SS1 and NT2 strains ofH.pyloriof 1 000 μg/ml and 1 000 μg/ml.Honeysuckle had a MIC with respect to SS1 and NT2 strains ofH.pyloriof 12 500 μg/ml and 6 250 μg/ml.Sophora flavescens had a MIC with respect to SS1 and NT2 strains ofH.pyloriof 25 000 μg/ml.Conclusion Western medicine directly inhibited and eradicatedH.pylori.Western medicine and traditional Chinese medicine in combination had an increased rate ofH.pylorieradication.