益肾壮骨颗粒剂对人工关节生物因素松动的影响

The Cytokine Study of Yishenzhuanggu's Effect on the Artificial Joint Loosening

目的:探讨益肾壮骨颗粒剂对人工关节生物因素松动的影响。方法:随机选择准备行人工髋关节置换术患者20例。采集外周血,分离单个核细胞,每例标本分成5份。A组:仅单个核细胞,为正常对照组;B组:单个核细胞+骨水泥微粒,为模型组;C组:单个核细胞+骨水泥微粒+益肾壮骨低剂量组(3mg·mL-1);D组:单个核细胞+骨水泥微粒+益肾壮骨中剂量组(6mg·mL-1);E组:单个核细胞+骨水泥微粒+益肾壮骨高剂量组(12mg·mL-1)。培养48 h后,用ELISA测定细胞上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量。结果:模型组的TNF-α和IL-6含量均明显高于正常对照组(P<0.01);单个核细胞+骨水泥微粒+益肾壮骨各剂量组的TNF-α和IL-6含量明显低于模型组(P<0.05)。结论:骨水泥微粒可刺激单个核细胞分泌TNF-α和IL-6,而益肾壮骨颗粒剂能有效地抑制单个核细胞分泌TNF-α和IL-6。益肾壮骨颗粒剂对人工关节生物因素松动方面有一定的防治作用。

Objective：To explore the effects of YiShenZhuangGu on aseptic cytokine of loosening of prothesis in vitro.Methods：From 20 patients,blood sample were taken,whose mononuclear cells were isolate and then divided into five groups.Group A：only mononuclear cells,which was control group;group B：mononuclear cells ＋ Poly methyl methacrylate particles;Group C：mononuclear cells ＋ Poly methyl methacrylate particles ＋ YiShenZhuangGu（3mg·mL-1）;Group D：mononuclear cells ＋ Poly methyl methacrylate particles ＋ YiShenZhuangGu（6mg·mL-1）;Group E：mononuclear cells ＋ Poly methyl methacrylate particles ＋ YiShenZhuangGu（12mg·mL-1）.They were cultured for 48 hours,and the a mount of osteolytic factors such as TNF-a and IL-6 of each group was determined.Results： The levels of TNF-α and IL-6 were much higher in group B than A,C,D and E（P0.01）.The levels of TNF-α and IL-6 were much lower in group C,D and E than B（P0.05）.Conclusion： It shows the Poly methyl methacrylate particles could stimulate mononuclear cells to excrete TNF-α and IL-6,which can be inhibited by YiShenZhuangGu.By inhibiting bone resorption associated with the particles and TNF-α and IL-6,YiShenZhuangGu may have clinical utility in the prevention and treatment of aseptic loosening of prothesis.