微波辅助萃取法提取菟丝子总黄酮的最佳工艺

Optimal Extraction Technology of Total Flavonoids from Cuscuta Chinensis by Microwave-Assisted Extraction

设计单因素和正交试验,采用微波快速反应系统对菟丝子进行提取,以芦丁为对照品,采用紫外分光光度法测定菟丝子中总黄酮的含量,以总黄酮的含量为考察指标,考察微波功率、提取时间、提取温度、乙醇浓度及料液比对总黄酮提取量的影响,优选最佳提取工艺。结果表明,微波辅助萃取法提取菟丝子总黄酮的最佳工艺条件为:乙醇体积分数70%、提取温度80℃、提取时间30min、料液比1∶20(g/mL)。

The total flavonoids was extracted from Cuscuta chinensis by microwave-assisted extraction.The influence of microwave power,temperature,time,solid-liquid ratio and alcohol concentration on extracting total flavonoids from Cuscuta chinensis by microwave-assisted extraction were explored by single factor experiments,and the optimum technology were determined by orthogonal experiments.The contents of total flavonoids were analyzed by ultraviolet spectrophotometry.The results show that the optimal conditions are as follows：1∶20（g/mL） of solid-liquid ratio,alcohol concentration of 70%,temperature at 80℃ and extraction time of 30 minutes.