摘要
采用紫外-可见光谱法、荧光光谱法,研究了两种新颖的席夫碱3-溴-5-氯水杨醛缩邻氯苯胺(BCSC)、3-溴-5-氯水杨醛缩对甲基苯胺(BCST)与DNA作用模式,研究表明两种化合物与CT-DNA作用紫外-可见光谱出现减色效应,随着CT-DNA的加入,化合物的荧光强度减弱,同时进行了KI对其荧光的猝灭实验,所有结果显示两种化合物与CT-DNA以嵌入模式结合。
The modes of the interaction of two novel Schiff base for BCSC and BCST with CT-DNA were studied by UV-Vis and fluorescence spectrometry.Experimental data indicated that the binding of the two compounds with CT-DNA would cause hypochromic effect in UV-Vis spectra,and the fluorescence intensity of compounds decreased with the addition of CT-DNA;meanwhile the quenching for the compounds with CT-DNA by KI were also studied.The results showed that the interactive model between the compounds and CT-DNA was an intercalative one.
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第2期826-831,共6页
Chinese Journal of Spectroscopy Laboratory
基金
植物化学陕西省重点实验室(宝鸡文理学院)资助项目(09JS068)
