苦瓜中维生素C含量的测定

Determination of Vitamin C in Bitter Gourd

建立苦瓜中维生素C含量直接测定新方法。以10%盐酸溶液为溶剂,采用紫外分光光度法测定含量。维生素C在0—10μg/mL浓度范围内与吸光度呈良好的线性关系,回归方程为:y=0.04746C+0.00109,r=0.9997。检出限为0.023μg/mL,加标回收率在94.8%—101.1%之间。该法操作简单,干扰离子少,结果准确,测量快速、准确度和灵敏度高,可应用于维生素C含量的测定。

The amount of vitamin C in bitter gourd was determined directly by UV spectrophotometry with 10% hydrochloric acid as solvent.The absorbance of vitamin C shows a linear relationship with the concentration in the range of 0—10μg/mL with a regression equation of y=0.04746C＋0.00109,r=0.9997.The detection limit is 0.023μg/mL,recovery is between 94.8%—101.1%.This method is simpler and less interference ion,accurate measurement of rapid,high accuracy and sensitive,and was applied to the determination of vitamin C.