一种新的测定诺氟沙星的化学发光分析法

A New Method for Determination of Nofloxacin by Chemiluminescence Analysis

基于在碱性条件下,诺氟沙星(NFLX)对鲁米诺-过氧化氢(Luminol-H2O2)化学发光体系的化学发光信号有强烈的抑制作用,建立了一种简单、快速、可连续测定诺氟沙星的流动注射化学发光分析新方法。该方法线性范围为7.0×10-8—1.0×10-6mol/L,检出限(3σ)为1×10-8mol/L,对3.0×10-7mol/L的诺氟沙星平行测定9次,相对标准偏差为0.9%。

Nofloxacin can greatly restrain the emission intensity of the chemiluminescence reaction between luminol and hydrogen peroxide in alkaline medium.A simple,rapid and continuous new method for the determination of nofloxacin was developed by chemiluminescence analysis with flow injection.The linear range was 7.0×10-8—1.0×10-6mol·L-1 and the detection limit（3δ） was 1×10-8mol·L-1.The relative standard deviation（n=9,Cnofloxacin=3.0×10-7mol·L-1） was 0.9%.