流动注射化学发光法测定山奈酚

Determination of Kaempferol with Flow Injection Chemiluminescence

采用流动注射技术,研究了高锰酸钾-山奈酚-过氧化氢体系的化学发光行为,对影响化学发光强度的因素进行了试验,建立了流动注射化学发光法测定山奈酚的新方法。方法检出限为1×1-0 10g.mL-1,线性范围为1.0×10-7—1.0×1-0 4g.mL-1。对1.0×1-0 6g.mL-1的山奈酚平行测定11次,其RSD为2.1%。该方法用于三叶青中山奈酚含量的测定,结果令人满意。

Chemiluminescence reaction of potassium permanganate-kaempferol-perhydrol system was investigated with flow injection technique.The effect of factors on chemiluminescence intensity was tested,and a new method for determination of trace kaempferol was established with flow injection chemiluminescence.The linear range for kaempferol was between 1.0×10-7g·mL-1 and 1.0×10-4g·mL-1 with the detection limitation of 1×10-10g·mL-1,and the relative standard deviation was 2.1% for kaempferol concentration of 1.0×10-6g·mL-1 for 11 repeated measurements.The method is applied to determine the content of kaempferol in radix tetrastigme with satisfactory results.