急性放射性肺损伤肺组织CD36表达及意义

Expression and Significance of CD36 on Acute Radiation-induced Lung Injury

采用健康雄性Wistar大鼠,6 MV X射线单次全胸野照射15 Gy,于照后不同时间HE和Masson染色观察大鼠肺组织的病理改变,免疫组化方法分析凝血酶敏感蛋白-1受体CD36在肺组织中的表达,以探讨放射性肺损伤大鼠肺组织病理和CD36在不同时间段的表达和意义。结果表明,HE和Masson染色提示照射后的1周肺泡腔有炎性细胞渗出,继之间质水肿,4及8周出现肺泡腔变小甚至结构破坏,局部实变,肺间质出现胶原纤维;CD36免疫组化标记显示:照射组在照后的第1、2、4、8周时间段CD36表达均明显强于对照组(p<0.01)。以上结果说明CD36参与了放射性肺损伤的发生发展过程,阻抑其表达可能对放射肺损伤有防治作用。

To investigate the pathology of acute radiation-induced lung injury and expression and significance of thrombospondin-1（TSP-1） receptor CD36 during different phases in rats,forty Wistar rats were randomly divided into control group（C） and radiation group（R）.Both groups were radiated with 6WV X ray linear accelerator at dose of 15 Gy thoracically,2 Gy/min,with SSD 1 m and radiation area 4.5 cm×4.5 cm.The pathological change of lung tissue and the expression of CD36 were detected with HE,Masson and immunohistochemistry staining,respectively.Result HE and Masson staining showed that only one week after radiation,the alveolar spaces had exudative inflammatory cells,following interstitial edema,and at 4 and 8 weeks,alveolar spaces shrank,even its structure was destroyed with local consol,and pulmonary interstitium appeared collagen fibers.The expression of CD36 was significantly higher in radiation group at all phases（p0.01）.The expression of CD36 increased significantly in radiation-induced lung injuy.CD36 participated in the process of radation-induced lung injury,and inhibition of CD36 expression may have preventive effect on radation-induced lung injury.