利用响应面法优化海洋细菌YDX-1产纤维素酶的发酵条件

Optimization of cellulase production conditions by marine bacteria YDX-1 using response surface methodology

采用响应面法对海洋细菌YDX-1产纤维素酶的发酵条件进行了优化。首先用Plackett-Burman法从8个因子中筛选出3个主要影响YDX-1产酶的因素,然后进行最陡爬坡实验逼近最佳响应面区域,最后通过Box-Behnken响应面设计实验得到最适条件为温度31℃、羧甲基纤维素钠(CMC-Na)41g/L、(NH4)2SO44.7g/L。在最适条件下测得的酶活为273.20U/mL,较优化前的150.13U/mL提高了81.97%。

Response surface analysis was applied to optimize the fermentation conditions for cellulase production by marine bacteria YDX-1. Firstly,two-level factorial design of Plackett-Burman was used to evaluate the effect of eight related factors on produced cellulase activity,and three main factors were screened out. Then the path of steepest ascent was taken to approach the optimal region of fermentation conditions. Finally,the optimal combined conditions for maximum enzyme activity were further optimized by Box-Behnken surface response design methodology and were determined as follows：temperature 31 ℃,CMC-Na 41 g/L,（NH4） 2SO4 4.7 g/L. Under the optimization of culture conditions,the cellulase activity increased from 150.13 U/mL to 273.20 U/mL,which was 81. 97% higher than preliminary culture.