螺旋藻培养过程中的营养盐监测与消耗

Determination and consumption of nutrient salts in Spirulina platensis cultivation

通过离子交换-抑制电导法,监测螺旋藻培养过程主要营养盐的消耗,同时考察了生物量累积与营养盐消耗的关系。实验结果表明,螺旋藻培养过程中营养源消耗量为:N源(NO-3)>P源(HPO2-4)>S源(SO2-4),基本不消耗Cl-。培养过程中的营养盐消耗符合Doseresp模型,实际值与模型拟合相关系数R2达到0.98以上。螺旋藻生物量干质量对NaNO3(YX/N)、K2HPO4(YX/P)、K2SO4(YX/S)的得率系数分别为1.86、21.30、37.02g/g。合理监测螺旋藻培养过程中的营养盐消耗,有利于控制藻的生长和质量,为补料策略的制定提供理论依据。

A method was established to determine nutrient consumption in Spirulina platensis cultivation using ion chromatography with suppressed conductivity detection. The relationship between biomass accumulation and nutrient salt consumption was studied. The nutrient consumption in Spirulina platensis cultivation was found to be in the following order：nitrogen source（NO-3） phosphorus source（HPO2-4） sulfur（SO2-4） . All of the nutrient salts consumption was fit to Doseresp model. The correlation coefficient between the actual values and fitted values was above 98%. The biomass yield against sodium nitrate,dipotassium phosphate and potassium sulfate was 1.86,21.3,37.02 g/g,respectively. Reasonably monitoring nutrient consumption in Spirulina platensis cultivation not only helps to control the growth and quality of algae,but also provides the theoretical basis for feeding strategies.