麻疯树籽油提取植物甾醇的研究

Phytosterol extraction from Jatropha seed oil

采用溶剂萃取法从麻疯树籽油中提取植物甾醇。通过单因素实验和正交实验优化提取工艺。结果表明,影响植物甾醇提取的因素主次顺序为:氢氧化钾乙醇溶液体积>皂化温度>氢氧化钾乙醇溶液浓度>皂化时间,最佳提取条件为:皂化温度90℃,皂化时间1h,氢氧化钾乙醇溶液体积15mL,氢氧化钾乙醇溶液浓度1.25mol/L。在此条件下,植物甾醇的提取率为121.3mg/10g。

Phytosterol was extracted from crude Jatropha seed oil with solvent-extraction method. The optimal conditions were obtained by single-factor and orthogonal test. The results indicated that the significance of the effects of these factors on the phytosterol extraction efficiency decreased in the order：volume of ethanol solution of potassium hydroxide,saponification temperature,concentration of potassium hydroxide alcohol solution,saponification time. The optimal extraction condition：saponification temperature of 90 ℃,saponification time of 1 hour,15 mL of ethanol solution of potassium hydroxide,1.25 mol/L of potassium hydroxide alcohol solution. Under these conditions,the extraction rate of plant sterols is 121.3 mg/10g.