三聚氰胺在大菱鲆体内的代谢消除规律研究

Metabolism and elimination of melamine in turbot

采用LC-MS/MS法研究三聚氰胺在大菱鲆(Scophthalmus maximus)体内的代谢消除规律。三聚氰胺以5 g/kg浓度添加到饲料中,连续投喂大菱鲆30 d,停药后,对大菱鲆肌肉、肝脏、肾脏组织残留的三聚氰胺进行测定。结果表明:三聚氰胺能够在大菱鲆肌肉、肝脏、肾脏各组织中富集并消除,最高血药浓度由高到低依次为Cmax肝脏>Cmax肾脏>Cmax肌肉,药时曲线下面积AUC肝脏>AUC肾脏>AUC肌肉,三聚氰胺在肾脏中消除最快,肝脏次之,肌肉最慢。停止给药30 d后,肌肉中三聚氰胺的含量降至检出限以下。建议在本实验条件下,三聚氰胺在肌肉中的代谢周期为40 d。

We studied the metabolism and elimination of melamine in turbots.The melamine was fed to turbot at 5 mg/kg,and the melamine retained in muscle,liver and kidney was determined.The results indicate that melamine can be accumulated and eliminated in turbot muscle,liver,and kidney.The order of maximum residues of melamine from high to low was Cmax liverCmax kidneyCmax muscle,while the area under the curve was AUCliverAUCkidneyAUCmuscle.The elimination speed of melamine in various tissues followed the order of kidney muscle liver.Thirty days post the feeding of melamine,the melamine level in muscle was below the detection limit.