内生真菌发酵提取物和植物生长调节剂对大豆根际细菌多样性的影响

Effects of endophytically fungal extracts and plant growth regulators on soybean rhizosphere bacterial diversity

为了研究内生真菌发酵提取物和植物生长调节剂对大豆根际细菌多样性的影响,采用PCR-DGGE技术分析了各处理中不同发育期的大豆根际细菌群落变化。结果发现发酵提取液和植物生长调节剂能增加部分优势菌群的数量,但对根际细菌类群结构影响并不明显;生育周期也是影响根际细菌数量的重要因素。另外割胶测序发现优势菌群主要是Proteobacteria(变形菌门)、Acidobacteria(酸杆菌纲)、Nitrospira(硝化螺旋菌属)、Bradyrhizobium(慢生根瘤菌属)等,这些也都是大豆根际比较常见的细菌类群。

In order to study the effects of endophytically fungal extracts and plant growth regulators on soybean rhizosphere bacterial diversity,the dynamics of rhizosphere bacterial community in different developmental stages was studied based on PCR-DGGE approach.The results showed that they could increase some bacteria in populations,but the variation of rhizosphere bacterial diversity by fermenta-tion extracts and plant growth regulator was no obvious,and the growth stages as an important factor that affects the rhizosphere bacteria.In addition,we found that the advantage bacteria mainly is Pro-teobacteria,Acidobacteria,Nitrospira,Bradyrhizobium,etc.These are the common bacterial species in the root of soybean.