显微共聚焦拉曼检测中药中非法添加的化学降糖药

Confocal Raman microscopy identification of synthetic hypoglycemic drugs adulterated in Chinese traditional medicines

目的:建立检测中药中非法添加化学降糖药的拉曼光谱法。方法:采用显微共聚焦拉曼光谱法,使样品在显微镜下微区成像,逐点选择不同的点采集拉曼光谱图,并与对照品光谱图进行比对分析,判断是否非法添加。结果:在市场监督抽样的中药中检出非法添加盐酸二甲双胍,检出结果进一步得到HPLC-MS法验证。结论:显微共聚焦拉曼光谱可作为中药、保健食品中非法添加化学药物检测的可选方法。

Objective： To develop a method of Raman spectroscopy for the identification of Synthetic Hypoglycemic Drugs adulterated in Chinese traditional medicines.Methods： The spectra of the samples,collected by confocal Raman microscopy with the spatial mapping and multivariate analysis of Raman maps,were compared with those obtained from the reference substances.Results： The synthetic hypoglycemic drugs such as metformin hydrochloride adulterated in the products supplied for inspection were rapidly differentiated.The results were further demonstrated by HPLC-MS analysis.Conclusion： Confocal Raman microscopy can be used as an alternative method for the identification of the adulteration of Chinese traditional medicines and health-care food with synthetic drugs.