部分粉葛品种遗传关系的SRAP研究

Analysis of genetic relationships of Pueraria thomsonii based on SRAP markers

目的:粉葛品种间遗传关系的分析。方法:以野葛和苦葛为外组,18个粉葛栽培品种为试材,通过SRAP分析,利用TREECONW软件分析遗传距离,UPGMA方法聚类,构建聚类树状图。结果:22对引物组合共得到338条扩增条带,其中有216条呈现多态性,占63.9%,平均每对引物组合产生15.4个位点和9.8个多态性位点,揭示了粉葛较为丰富的遗传多样性。粉葛品种遗传距离变化范围在0.004 7～0.265 8,平均0.136;聚类结果显示18个粉葛品种及外组被明显地划分三大聚类群,大部分品种并没有按地域形成独自的类群。结论:SRAP标记为粉葛遗传关系研究提供了新的DNA分子标记技术手段。

Objective：To determine the interspecies relationships of 18 Pueraria thomsonii cultivars in molecular level.Method：Eighteen P.thomsonii cultivars were evaluated by using sequence-related amplified polymorphism（SRAP） markers,with P.lobata and P.peduncularis as contrast species.Systematic relationships were constructed based on the UPGMA method by TREECONW software.Result：The results showed that 22 primer pairs produced 338 loci,out of which 216 were polymorphic,the percentage of polymorphic loci was 63.9%.An average of 15.4 loci and 9.8 polymorphic loci were generated by each pair of primers.Genetic distance was analyzed by TREECONW software.Genetic distance of 18 P.thomsonii were changed from 0.004 7 to 0.265 8,with an average of 0.316.Using cluster analysis（UPGMA） based on those polymorphism bands amplified with SRAP primers,the 22 cultivars were classified into three groups,groups 1 with 18 P.thomsonii,group 2 with 3 P.lobata,and group 3 with 1 P.peduncularis.Most of the P.thomsonii from the same region were not in the same group.Conclusion：SRAP markers could be a good marker for genetic relationship research in the P.thomsonii.