多指标正交试验优化白首乌C_(21)甾总苷的醋酸水解条件

Optimization of the hydrolysis process for C_(21) steroidal glycoside of Bai Shou Wu by acetic acid with multi-target orthogonal design

目的:研究白首乌C21甾总苷醋酸水解的最佳条件。方法:以开德苷元-3-O-β-D-洋地黄毒糖苷、告达庭、开德苷元-3-O-α-L-迪吉糖-(1→4)-β-D-磁麻糖苷和告达庭-3-O-β-D-磁麻糖苷的含量为指标,采用正交试验设计L9(33)进行优选,考察水解温度(A)、水解时间(B)、醋酸浓度(C)3个因素对白首乌C21甾总苷醋酸水解条件的影响,同时建立HPLC含量测定的方法。结果:影响醋酸水解的主次因素为A>B>C,最佳水解条件为5.0%醋酸溶液,100℃回流6 h。结论:此水解条件明显提高了白首乌中抗癌有效成分的含量,对研究抗肿瘤药物具有重要作用。

Objective：To study the optimizal hydrolysis process for C21 steroidal glycoside of Bai Shou Wu by acetic acid.Method：The effects of acetic acid concentration,reaction temperature and reaction time had been investigated using orthogonal design and the contents of kidjoranin 3-O-β-digitoxopyranoside,caudatin,kidjoranin 3-O-α-L-diginopyranosyl-（1→4）-β-cymaropyranoside and caudatin 3-O-β-cymaropyranoside as response indexs were determined by the high performance liquid chromatography.Result：The factors influencing acetic extraction efficiency were as follows：ABC（A.reaction temperature;B.reaction time;C.acetic acid concentration）.The optimal hydrolysis condition obtained was：refluxing for 6 hours with 5.0% dilute CH3COOH solution at 100 ℃.Conclusion：The content of antitumor active ingredients under the optimum hydrolysis condition is raised obviously and has a great part in studying this antitumor drug.