HPLC测定藏药螃蟹甲中5个环烯醚萜苷的含量

Determination of five iridoid glycosides in Phlomis younghusbandii by HPLC

目的:建立RP-HPLC同时测定藏药螃蟹甲中5种环烯醚萜苷的含量。方法:色谱柱为Symmetry C18(4.6mm×150 mm,5μm),流动相为乙腈(A)-水(B)梯度洗脱,0～5 min,7%A,5～10 min,7%～12%A,10～40 min,12%A;流速1.0 mL.min-1;柱温20℃,检测波长235 nm。结果:sesamoside,山栀苷甲酯,7,8-dehydropenstemoside,penstemoside和8-O-乙酰山栀苷甲酯分别在0.050～0.650(r=0.9993),0.050～0.350(r=0.9995),0.040～0.280(r=0.9994),0.010～0.070(r=0.999 6),0.040～0.280(r=0.999 7)g.L-1与峰面积线性关系良好;平均加样回收率均96%～104%,RSD均小于5.0%(n=6)。结论:此方法简便、准确,重复性好,结果稳定,可作为螃蟹甲药材的质量控制方法。

Objective：To develop an RP-HPLC method for the determination of five iridoid glycosides in Phlomis younghusbandii.Method：HPLC analysis was performed on a Symmetry C18（4.6 mm×150 mm,5 μm,Waters） column eluted with acetonitrile（A） and water（B） in gradient elution.The gradient program was as follows：0-5 min kept 7% A;5-10 min changed to 12% A;10-40 min kept 12%A.The flow rate was 1.0 mL·min-1.The column temperature was 20 ℃ and the detection wavelength was 235 nm.Result：The linear ranges of sesamoside,shanzhiside methyl ester,7,8-dehydropenstemoside,penstemoside and 8-O-acetylshanzhiside methyl ester were 0.050-0.650（r=0.999 3）,0.050-0.350（r=0.999 5）,0.040-0.280（r=0.999 4）,0.010-0.070（r=0.999 6）,0.040-0.280（r=0.999 7） g·L-1,respectively.The average recoveries（n=6） of them were all between 96% and 104%,RSD5.0%.Conclusion：The method is simple,accurate,repeatable and stable,which can be used for quality control of P.younghusbandii.