HPLC测定大鼠血浆和房水中的芒果苷

Determination of mangiferin in rat plasma and aqueous humor by HPLC

目的:建立了测定大鼠血浆和房水中芒果苷含量的液相色谱检测方法。方法:以对硝基苯酚作内标,甲醇-2%冰醋酸(40∶60)为流动相,流速为1.0 mL.min-1。在Cosmosil ODS C18色谱柱(4.6 mm×250 mm,5μm)上进行分离。结果:方法的线性范围分别为血浆0.50～250.00 mg.L-1,房水0.10～10.00 mg.L-1,线性回归系数均在0.995 4以上,日内和日间精密度RSD均小于12%,最低检量限0.05 mg.L-1。结论:本方法具有简便、准确等优点,可满足芒果苷在大鼠血浆和房水中药动学研究的需求。

A HPLC method was developed for the determination of mangiferin in rat plasma and aqueous humor.4-Nitrophenol was used as internal standard.Analysis was performed on a Cosmosil ODS C18 analytical column（4.6 mm×250 mm,5 μm） with mobile phase consisting of methanol-water（40∶60） with 2% glacial acetic acid at a flow rate of 1.0 mL·min-1.The calibration curve of mangiferin in rat plasma and aqueous humor showed excellent linear behaviors over the investigated concentration of 0.50-250.00 mg·L-1 in plasma and 0.10-10.00 mg·L-1 in aqueous humor,respectively,and the correlation coefficients were all above 0.995 4.The intra-day and inter-day precisions for all samples were measured to be below 12%.The limit of quantitation was 0.10 mg·L-1 and low enough for the determination of mangiferin in all samples.The validated method has been successfully applied to preliminary pharmacokinetics study of mangiferin in rat plasma and aqueous humor.