联合检测P53及雌孕激素受体在评估子宫内膜癌预后中的意义

Significance of combined detection of P53 and sexural hormones receptors for assess the prognosis in patients with endometrial carcinoma

目的探讨联合检测P53蛋白的表达及雌孕激素受体在子宫内膜癌中的表达及临床意义。方法应用免疫组织化学Envision法检测52例子宫内膜癌中ER、PR和p53的表达情况并进行统计学分析。结果 ER、PR和p53阳性表达率分别为51.9%、50.0%和46.2%。ER、PR的阳性表达率与癌组织的细胞分化程度有关(P<0.05)。随着组织学分级的增高,ER、PR阳性表达率逐渐降低(在Ⅰ、Ⅱ和Ⅲ级子宫内膜癌中ER阳性表达率分别为64.0%、56.3%和18.2%,PR阳性表达率分别为72.0%、37.5%和18.2%)。随着组织学分级的增高及淋巴结转移,p53的阳性表达率逐渐增高(P<0.05),p53表达与ER、PR表达有关(P<0.05)。结论 ER、PR和p53的表达与子宫内膜癌组织学分级、类型和生物学行为密切相关,其测定对评估子宫内膜癌预后,指导临床治疗具有重要意义。

【Objectives】 To explore the clinical significance of combined detection of P53 protein exppresion and sexural hormones receptors in patients with endometrial carcinoma.【Methods】 The expression of ER,PR and P53 in 52 cases of endometrial carcinoma was detected by Envision immunohistochemical method and the experiment data were statistically analyzed.【Results】 The positive expression rates of ER,PR and P53 in 52 cases of endometrial carcinoma were 51.9%,50.0% and 46.2%,respectively.The positive rates of ER and PR were related to the degree of differentiation of tumor（P 0.05）.The positive rates of ER and PR in endometrial carcinoma were gradually decreased with histological grade developed（ER was expressed in 64.0%,56.3% and 18.2% of endometrial carcinoma in grade Ⅰ,Ⅱand Ⅲ,while PR in 72.0%,37.5% and 18.2%,respectively）.The positive rate of P53 in endometrial carcinoma increased gradually with histological grade development and lymph node metastasis（P 0.05）.The expression of p53 related with the expression of ER and PR（P 0.05）.【Conclusion】 The expression of ER,PR and p53 significantly correlated with histological grades,types and biological behaviors of the endometrial carcinoma.The detection of these molecular proteins may be benefit for prognosis evaluation and the patient＇s management.