摘要
目的研究羟乙基淀粉130/0.4(万汶)对重症急性胰腺炎大鼠肝脏水通道蛋白-1(AQP-1)表达及肝脏损伤的影响。方法将雄性SD大鼠48只按完全随机法分为假手术组(shamoperation group,Sham组)、重症急性胰腺炎组(severe acute pancreatitis,SAP组)、羟乙基淀粉治疗组(HES组),每组分6h、24h两个时间点,每个时间点8只。以5%牛磺脱氧胆酸钠逆行胰胆管注射建立SAP模型。各组于造模后6h、24h处死大鼠,检测血淀粉酶(AMY),谷丙转氨酶(ALT),谷草转氨酶(AST),肝脏含水量,HE染色观察胰腺、肝脏组织病理,酶联免疫吸附(ELISA)方法检测血清TNF-α水平,RT—PCR检测肝组织AQP—lmRNA,免疫组化检测肝组织AQP-1蛋白。结果各时间点SAP组与假手术组比较,血淀粉酶、ALT、AST、肝脏含水量、血清TNF—α水平、AQP—lmRNA及AQP-1蛋白均显著上调(P〈O.05);HES组与SAP组比较,血淀粉酶、ALT、AST、肝脏含水量、血清TNF_a水平、AQP-1mRNA及AQP-1蛋白均显著下调(P〈0.05)。6h时间点,Sham组、SAP组和HES组AQP-1mRNA表达量分别为(0.402±0.023)mmol/L、(0.811±0.032)mmol/L和(0.595±0.015)mmol/L,3组间差异有统计学意义(P〈0.05);24h时间点,各组AQP-lmRNA表达量分别为(0.412±0.017)mmol/L、(0.823±0.029)mmol/L和(0.607±0.021)mmol/L,3组间差异有统计学意义(P〈0.05)。6h时间点,Sham组、SAP组和HES组AQP-1蛋白表达分别为(2.07±0.25)、(6.90±0.38)和(4.48±0.29),3组间差异有统计学意义(P〈0.05);24h时间点,各组AQP-1蛋白表达分别为(2.32士o.31)、(7.04±0.32)和(4.56±0.25),3组间差异有统计学意义(P〈0.05)。胰腺、肝脏组织病理HES组较SAP组明显改善。结论HES130/0.4可一定程度改善重症急性胰腺炎的肝脏损伤,减轻毛细血管渗漏,AQP-1在重症急性胰腺炎肝脏损伤导致的毛细血管渗漏中起一定作用。
Objective To investigate the effect of hydroxyethyl starch (HES 130/0.4) on the expression of aquaporin 1 (AQP-1) and liver injury in rats with severe acute pancreatitis(SAP). Methods Forty-eight male SD rats were randomly divided into three groups: Sham, SAP, and HES; each group was divided into 6 hour and 24 hour timepoints, with 8 in each subgroup. An SAP model was induced by injecting 5% sodium taurodeoxycholate into the biliary pancreatic duct. AMY, ALT, AST and water content in the liver were measured and TNF-a was examined by ELISA, and the ex- pression of liver AQP-lmRNA was determined by RT-PCR, and the expression of liver AQP-1 protein was evaluated by immunohistochemical methods. Results Compared with the Sham group, the level of AMY, ALT, AST, TNF-a, water content, AQP-lmRNA and AQP-1 protein increased signifi- cantly in the SAP group (P〈0.05). Compared with the SAP group, the level of AMY,ALT,AST, TNF-a, water content, AQP-lmRNA and AQP-1 protein decreased significantly in the HES group (P〈0.05). At 6 hours, the expressions of liver AQPdmRNA were (0. 402 4-0. 023), (0. 811 ± 0. 032) and (0. 595 ± 0. 015) in the Sham, SAP, and HES groups, respectively; at 24 hours, they were(0. 412±0. 017), (0. 823 ± 0. 029) and (0. 607± 0. 021 ), with significant differences between each group (P〈0.05). At 6 hours, the expressions of liver AQP-1 proteins were (2.07 ± 0. 25), (6.90±0.38)and (4.48±0.29) in the Sham, SAP and HES groups, respectively;at 24 hours, they were (2.32±0.31), (7. 04±0. 32) and (4. 56± 0. 35), with significant differences between each group (P〈0.05). Compared with the SAP group, the pathology of the pancreas and liver ameliorated significantly in the HES group. Conclusions Hydroxyethyl starch 130/0.4 may ameliorate liver injury of severe acute pancreatitis and alleviate the capillary leak. AQP 1 may play a role in the capillary leak caused by the liver injury of severe acute pancreatitis.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2011年第2期123-126,共4页
Chinese Journal of Hepatobiliary Surgery