消癌解毒方抑制肝癌H22移植瘤的生长及其机制

Xiaoai Jiedu Recipe inhibits transplanted hepatocarcinoma H22 tumors and the related mechanism

目的:研究消癌解毒方(Xiaoai Jiedu Recipe,XJR)对肝癌H22细胞小鼠移植瘤的抑制作用及其可能的机制。方法:建立小鼠H22移植瘤模型,分对照组和XJR低、中、高剂量(10、30、90g/kg)组及顺铂(0.001g/kg)组进行治疗,检测各组移植瘤生长情况,H-E染色观察各组移植瘤组织的病理改变。流式细胞术检测各组移植瘤细胞周期及凋亡率,ELISA法测定各组移植瘤小鼠外周血VEGF水平。结果:与对照组比较,XJR各剂量以及顺铂对H22移植瘤的生长均具有显著的抑制作用,抑瘤率分别为24.5%、42.8%、21.1%、58.6%(P<0.05或P<0.01)。病理观察见中剂量XJP组和顺铂组移植瘤组织大片状坏死,有明显染色质固缩环。中剂量XJR和顺铂将移植瘤组织细胞阻滞于G0/G1期(P<0.05),S期细胞数明显减少(P<0.05)。中剂量XJR组移植瘤细胞的凋亡率与顺铂组相当[(60.52±6.40)%vs(71.32±16.02)%,P>0.05]。中、高剂量XJR组和顺铂组小鼠外周血VEGF水平均显著降低[(104.3±6.1)、(105.8±7.2)、(88.6±4.3)vs(120.7±12.6)ng/ml,P<0.05或P<0.01]。结论:XJR能抑制H22小鼠移植瘤的生长,促进移植瘤细胞凋亡、抑制VEGF的产生可能是其抗癌作用机制之一。

Objective：To study the inhibitory effects of Xiaoai Jiedu Recipe（XJR） against transplanted hepatocarcinoma H22 tumors in mice and the related mechanism. Methods：H22-transplanted tumor mouse models were established and were divided into control group,XJR treatment groups （10,30,90 g/kg）,and cisplatin treatment group. The growth of tumors in different groups was measured,and pathology changes of transplanted tumor tissues in different groups were examined by H-E staining. Flow cytometry was applied to examine cell cycle and sub-diploid apoptosis rate of transplanted tumor cells,and VEGF levels in the peripheral blood of mice were measured by ELISA. Results：Compared with control group,different XJR groups and the cisplatin group significantly inhibited growth of H22-transplanted tumors （24.5%,42.8%,21.1,58.6% vs 0,P〈0.05,P〈0.01）,cells in XJR groups showing massive necrosis and obvious chromosome condensation. Medium-dosage XJR and cisplatin blocked transplanted tumor cells in G0/G1 phase （P〈0.05）,and the cells in S phase were significantly reduced （P〈0.05）. The sub-diploid apoptosis rate of transplanted tumor cells in medium-doseage XJR group was similar to that in cisplatin group （60.52±6.40 vs 71.32±16.02,P〉0.05）. Peripheral VEGF levels were significantly lower in the medium-,large-dosage XJR groups and cisplatin group than in the control group （104.3±6.1,105.8±7.2,88.6±4.3 vs 120.7±12.6,P〈0.05,P〈0.01）. Conclusion：Xiaoai Jiedu Recipe can inhibit the growth and promote apoptosis of H22-transplanted tumor cells,and inhibition of VEGF may be one of the mechanisms for the anticancer effect.