电针对雌激素调节生殖内分泌功能的作用及机制研究

Influence of Electroacupuncture on Efficacy of Estrogen in Regulating Hypothalamic Reproductive Endocrine Activity in Rats

目的:研究针刺对药物调节生殖内分泌功能的作用效应。方法:以去卵巢SD大鼠为模型,通过实时定量聚合酶链反应及酶联免疫吸附法分析正常组、模型组、针刺关元组、雌激素组(0.5 mg/kg)、针刺关元+小剂量雌激素组(0.25 mg/kg)下丘脑促性腺激素释放激素(GnRH)、G蛋白耦联受体54(GPR54)、肿瘤转移抑制因子(Kiss)-1、促肾上腺皮质激素释放激素(CRH)mRNA的表达及外周血雌激素、皮质醇水平。结果:模型组与正常组相比,下丘脑GnRH、GPR54、Kiss-1 mRNA的表达明显增高(P<0.05),下丘脑CRHmRNA的表达明显降低(P<0.05),血清皮质醇及雌激素水平明显降低(P<0.05)。关元+小剂量雌激素组、雌激素组与模型组相比,GnRH、GPR54、Kiss-1 mRNA的表达明显降低(P<0.05),CRH mRNA的表达明显增高(P<0.05),血清皮质醇及雌激素水平增高(P<0.05)。关元组与模型组相比,GnRH mRNA的表达明显降低(P<0.05),GPR54、Kiss-1 mRNA的表达有降低的趋势(P>0.05),CRH mRNA的表达有增高的趋势(P>0.05),血清皮质醇及雌激素水平有增高的趋势(P>0.05)。结论:电针能促进小剂量雌激素对生殖内分泌功能的调节作用。

Objective To observe the effect of electroacupuncture （EA） on the efficacy of estrogen-induced regulation of reproductive endocrine in ovariectomized （OVX） rats. Methods Thirty SD rats were randomly divided into control, model （OVX）, EA-Guanyuan（CV 4）, Estrogen （E） and EA＋E groups （n = 6 in each group）. OVX model was duplicated by removing the bilateral ovaries. EA （5 Hz/20 Hz, 1 -2 mA） was applied to ＂Guanyuan＂ （CV 4） for 30 min, once every other day and for 3 sessions. Estrogen at doses of 0.5 mg/kg and 0.25 mg/kg was intraperitonealy given to rats in the E group and EA＋ E group, respectively. Serum estrogen and cortisol contents were assayed by enzyme linked immunosorbent assay and the expression of hypothalamic gonadotrophin releasing hormone（GnRH） mRNA, G protein-coupled receptor 54 （GPR 54） mRNA, kisspeptin-1 （Kiss-1） mRNA and corticotropin releasing hormone （CRH） mRNA were detected by using real time polymerase chain reaction （RT-PCR）. Results Compared with the control group, the expression levels of GnRH mRNA, GPR 54 mRNA and Kiss-1 mRNA in hypothalamus in the model group were increased significantly （P〈0.05）, while the expression of hypothalamic CRH mRNA was down-regulated obviously （P〈0.05）, and serum cortisol and estrogen levels were reduced significantly （P〈0.05）. In comparison with the model group, the expression levels of hypothalamic GnRH mRNA in the EA, E and EA＋ E groups, and those of hypothalamic GPR 54 mRNA and Kiss-1 mRNA in the E and EA-I- E groups were down-regulated considerably （P〈0.05） ; whereas hypothalamic CRH mRNA expression levels in the E and EA ＋ E groups were up-regulated obviously （P〈0.05）, and serum cortisol and estrogen contents in the E and EA＋ E groups were increased remarkably （P〈0.05）. Comparison among the EA and EA＋ E groups showed that the effects of EA＋E group were significantly superior to those of EA group in down-regulating hypothalalmic GnRH mRNA and GPR 54 mRNA expression, and in up-regulating hypothalamic CRH mRNA expression and serum cortisol and estrogen contents （P^0.05）. No significant differences were found between E and EA＋E groups in down-regulating hypothalamic GnRH mRNA, GPR 54 mRNA and Kiss-1 mRNA expression, and in up-regulating hypothalamic CRH mRNA expression and serum cortisol and estrogen levels （P〈0.05）. Conclusion EA-CV 4 is able to potentiate the effects of low-dose of estrogen in down-regulating hypothalamic GnRH mRNA,GPR 54 mRNA and Kiss-1 mRNA expression, and up-regulating hypothalamic CRH mRNA expression and raising serum cortisol and estrogen contents in OVX rats, showing a favorable modulation effect on reproductive endocrine activity.