光动力疗法及其联合注射卡介苗对小鼠肝癌移植瘤的抑瘤作用与免疫反应

Tumor-suppressive Effect and Immune Reaction of Photodynamic Therapy Combined with Local Injection of BCG on Liver Carcinoma Xenografts in Mice

目的探讨光动力疗法(PDT)联合肿瘤局部注射卡介苗(BCG)的局部免疫反应及抑瘤效应。方法建立小鼠Heps肝癌移植瘤模型96只,随机分为对照组、PDT组、BCG组和PDT+BCG联合组。观测各组移植瘤体积的变化。以F4/80抗体为标记,采用免疫组化法计数肿瘤局部巨噬细胞(MΦ)数量。结果 1.各治疗组治疗后7 d的移植瘤体积较对照组均明显缩小(P<0.01)。联合组治疗后7 d的肿瘤体积较单纯PDT组明显缩小(P<0.05)。2.免疫组化染色结果显示,PDT组和联合组治疗后2、24 h和7 d肿瘤局部MΦ数量均明显高于对照组和BCG组(P<0.01)。上述时间点联合组肿瘤局部MΦ数量明显高于PDT组(P<0.05)。结论 PDT使小鼠肝癌移植瘤体积缩小,增强荷瘤宿主治疗局部的免疫力。PDT联合局部注射BCG的治疗方法的疗效优于单纯PDT和单纯BCG。

Objective To invest the local immune tumor-inhibition effect of photodynamic therapy（PDT） combined with local injection of BCG.Methods Ninety-six tumor-bearing models were established with liver carcinomas subcutaneously in mice.The mice were divided randomly into：control group,PDT group,BCG group,and PDT plus BCG group.Tumor size was measured in each group.The expression of F4/80 antibody was detected by immunohistochemistry in order to count the macrophages（MΦ） in the local tumor.Results（1）The tumor volume of PDT group,BCG group,and PDT plus BCG group was smaller than that of the control group 7d after therapy（P0.01）.The tumor volume of PDT plus BCG group was smaller than that of the PDT group 7d after therapy（P0.05）.（2）2h,24h and 7d after therapy,immunohistochemical staining showed that the number of macrophages in the tumor of PDT group and PDT plus BCG group increased obviously（P0.01）,compared with the control group and BCG group.The number of macrophages in the tumor of PDT plus BCG group increased obviously compared with the PDT group（P0.05）.ConclusionsPDT has the inhibitory effect on tumor growth of Heps cell line xenografts in mice,and can spontaneously induce the local immune effects.The therapeutic effect of PDT combined with BCG is superior to that PDT only.