间日型猴疟血小板动态变化及药物干预观察

Dynamic changes of platelets during Plasmodium cynomolgi infection and after drug treatments in rhesusmonkeys

目的观察恒河猴感染间日型猴疟后原虫密度消长与PLT数值的关系及抗菌药物和抗疟药治疗后对PLT变化的影响。方法健康猴1只，经血液感染间日型猴疟后2d开始采血检杏疟原虫及PLT计数。发现疟原虫后24d，阿奇霉素治疗3d，总量为1500mg；发现疟原虫后30d，青蒿琥酯治疗5d，总量为194．4rng。观察治疗期间疟原虫与PLT变化规律。同时设健康对照猴1只。结果实验猴被感染疟原虫前PLT计数为240×10^9／L，感染后，当血疟原虫为2／100WBC时，PLT计数为540×10^9／L。感染猴原虫密度波动在（1～60）／100WBC，而PLT数值维持在（130～l50）×10^9／L之间。实验猴灌服阿奇霉素后，原虫密度波动在（16～64）／100WBC之间，而平均PLT汁数回升到234．5×10^9／L，接受青蒿琥酯治疗64h后血检未发现原虫，PI。T计数平均值已回升到247×10^9／L。结论疟原虫感染后PLT计数下降，阿奇霉素和青蒿琥酯治疗猴疟能较快提升PLT计数。

Objective To observe platelet dynamics in a monkey infected with Plasmodium cynomolgi hefore and after treatments with antibiotics and antimalarial drug. Methods One experimental monkey was examined for parasite density and platelet count 2 clays after parasite inoculation. Observation without treatment continued for 24 days after the parasite was detected in the blood sample of the monkey. Then the monkey was treated with Azithromycin （total 1500 rag） for 3 days. Thirty days after parasite detection in the blood, the monkey was treated with Artesunate for 5 days. Parasite density and platelet count were monitored daily during treatments. The result was compared with that from a healthy monkey as control. Results The experimental monkey＇s platelet count was 240X10^9/L before infection. When parasite density was 2/100 white blood cells （WBC）, platelet count increased to 540 × 10^9/L. During the subsequent period of infection, parasite density fluctuated at （1--60）/100 WBC, and the platelet count reduced to a persistent level of （130--150）X 10^9/I.. After the infected monkey was treated with Azithromycin, parasite density reduced initially but subsequently fluctuated at （15--64）/100 WBC. Meanwhile, platelet count was restored to 234.5 X 10^9/L. The infected monkey was treated with Artesunate and parasite clearance time was 64 hours, and the mean platelet count was 247 ×10^9/L after treatment. Conclusion Azithromycin and Artesunate treatment have direct influence on the recovery of platelet counts during malaria infection in monkeys.