电针刺激大鼠足三里穴对肝缺血再灌注损伤的保护作用

Electroacupuncture of Zusanli acupuncture point protects against hepatic ischemia/reperfusion injury in rats

目的探讨电针刺激大鼠足三里穴对肝缺血再灌注损伤的保护作用及可能机制。方法 40只雄性SD大鼠被随机分为假手术(A)组、肝缺血再灌注(B)组、非经络穴位电针刺激(C)组和足三里穴电针刺激(D)组,每组10只。B组不给予电针刺激;C、D两组于缺血前分别针刺非经络穴位和足三里穴,强度均为4mA、2/100Hz的电刺激,持续45min。B、C、D组以3cm血管阻断夹阻断左横叶及中叶的入肝血流,保留右叶及尾叶血供,造成70%的肝组织缺血,90min后恢复血供,再灌注后8h从下腔静脉抽血,采用全自动生化分析仪测定血清丙氨酸转氨酶(ALT);同时处死大鼠,以实时定量PCR法检测肝组织肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)等细胞因子mRNA的表达。结果 B、C、D组与A组比较,ALT、TNF-α和IL-10均有显著升高,差异具有统计学意义(P<0.05),D组ALT和TNF-α升高幅度均显著低于B和C组(P<0.05);而B、C、D组间IL-10差异无统计学意义(P>0.05)。结论电针刺激足三里穴能有效减轻肝脏缺血再灌注损伤,其作用机制可能与胆碱能抗炎通路有关。

Objective To study the protective effects of electroacupuncture of Zusanli acupuncture on rat hepatic ischemia/reperfusion injury and the possible mechanism. Methods Forty male SD rats were randomly divided into 4 groups （each group n=10）： sham（A）, ischemia/reperfusion（B）, ischemia/reperfusion ＋ non-electroacupunture（C）, and ischemia/ reperfusion ＋ electroacupuncture of Zusanli acupuncture point（D）. Animals in C and D groups received electroacupuncture of Zusanli acupuncture point （4 mA, 2/100 Hz） before ischemia for 45 min. Hepatic ischemia/reperfusion injury （70%） was induced by blocking blood inflow of the left transverse lobe and middle lobe for 90 rain, then reperfusion was allowed for 8 h; blood samples were obtained from the inferior vena cava for determination oI serum ALT. Meanwhile, real-time PCR was used to examine the heptaic expression of TNF-~ and IL-10. Results B, C, and D groups demonstrated significantly higher levels of serum ALT and hepatic TNF-~ and IL-10 compared with A group（P〈0.05）, and the increase of ALT and TNF-a in D group was significantly lower than those in B and C group （P^0.05）. Expressi6n of IL-10 was not significantly different between the 3 groups （P〈 0. 05）. Conclusion Electroacupuncture of Zusanli acupuncture point can effectively protect against hepatic ischemia/reperfusion injury, which is＂possibly related to the cholinergic anti-inflammatory pathway.