摘要
目的:探讨不同的血清饥饿方法和饥饿时间对人牙髓细胞(HDPCs)细胞周期的影响。方法:血清直接饥饿和梯度饥饿处理人牙髓细胞。倒置显微镜观察细胞形态;CCK-8检测血清饥饿处理对HDPCs增殖能力的影响;流式细胞术检测细胞周期验证HDPCs同步化的效果。结果:血清直接饥饿处理或梯度饥饿处理后其G0/G1期HDPCs的比例均显著高于一般培养组(P<0.05),但血清直接饥饿组和梯度饥饿组间无显著性差异(P>0.05)。用含5 mL/L FBS的DMEM培养基培养48 h能获得较满意的结果,G0/G1期细胞百分比达85.95%。结论:血清饥饿法能有效地使人牙髓细胞同步于G0/G1期;5 mL/L FBS血清饥饿处理细胞48 h可取得较好的细胞周期同步化效果。
AIM： To examine the effects of serum starvation on cell cycle synchronization of human dental pulp cells（HDPCs） by treating HDPCs with different starvation methods and starvation times.METHODS： HDPCs were treated with serum direct starvation and gradient starvation.The morphological changes of HDPCs were observed by an inverted microscope.CCK-8 assay was used to investigate the effects of serum starvation on cell proliferation,and cell cycle distribution of HDPCs was detected by flow cytometry（FCM）.RESULTS： HDPC growth was significantly inhibited by serum starvation.Treatment of HDPCs with serum direct starvation or gradient starvation resulted in a significant increase of cells arrested at G0/G1 stage in comparision with the control group（P0.05）,but there was no significant difference between the serum direct starvation and gradient starvation groups.HDPCs cultured in DMEM containing 5 mL/L fetal bovine serum（FBS） for 48h showed the most satisfactory result,with 85.95% of HDPCs at G0/G1 phase.CONCLUSION： Serum starvation can efficiently arrest HDPCs at G0/G1 stage,and the optimum result can be achieved with 0.5%FBS serum and a culture period of 48h.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2011年第2期67-71,共5页
Chinese Journal of Conservative Dentistry
基金
教育部博士点新教师基金项目(20094433120004)
广东省科技计划项目(2008B011300018-8)
